Recent publications
- How could the affordable, reliable and continuous production of lipid nanoparticles revolutionize healthcare? May 6, 2025No abstractLuke Burroughs
- Deprotonation-Induced Large Fluorescence Turn ON in a 2,4-Bis(benzo[<em>d</em>]thiazol-2-yl)phenol (HBT-BT) Derivative May 5, 20252-(2'-Hydroxyphenyl)benzothiazole (HBT) is an interesting candidate that has been used to develop fluorescent probes with large Stokes' shifts (Δλ > 100 nm) by utilizing excited-state intramolecular proton transfer (ESIPT). The efficiency of the ESIPT process in HBT is dependent on the acidity of the central phenolic group. The possible deprotonation of the enol form of […]Kavinda M Arachchige
- Understanding drug release mechanisms of risperidone in situ forming implant depots in rabbits April 28, 2025Injectable poly(lactic-co-glycolic acid) (PLGA)-based in situ forming implants are a promising long-acting drug delivery system. They are liquid formulations that solidify through solvent exchange to form drug-PLGA depots that become isolated from the surrounding tissue following injection. There have been limited studies focusing on in vivo drug release mechanisms for this dosage form. However, such […]Xiaoyi Wang
- Comparative pharmacokinetics of seven propargyl-linked antifolate antibiotics in the mouse April 10, 2025Antimicrobial resistance (AMR) to existing antibiotics poses a critical global health challenge, with significant morbidity and mortality from bacterial infections. Methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant strains (VISA, VRSA) are among the most pressing threats, particularly for vulnerable populations. To combat this crisis, the development of novel therapeutic strategies is imperative. We report the pharmacokinetic […]John Hoody
- Recent advancements and future applications of intrauterine drug delivery systems April 7, 2025INTRODUCTION: Intrauterine devices (IUDs) are drug-device combination products that are placed inside the uterus above the endometrium. IUDs contain a therapeutic agent used for contraception and have been shown to have other therapeutic benefits. Over the past century, intrauterine drug delivery systems (IUDDS) have revolutionized women's health, and they hold immense growth potential in the […]Ashwin Abhang
- Photophysical behaviour and sub-cellular specificity of a pyrene-benzothazolium imaging dye: A study of regio-effect April 6, 2025Pyrene-based small-molecule fluorescent dyes have been widely used in biomedical imaging and sensing applications. Among them, pyrene-based π-acceptor (π-A) type probes are an important class of fluorescent dyes due to their ability to produce a large Stokes' shift (Δλ >100 nm) by strong intramolecular charge transfer (ICT) process. In this work, a pyrene-benzothiazolium-based π-A probe […]Kavinda M Arachchige
- Aging-induced microstructural evolution in risperidone loaded PLGA microspheres March 30, 2025This study demonstrates the application of innovative imaging-based characterization techniques to quantify structural changes as a function of ageing for poly (lactic-co glycolic acid) (PLGA) microspheres. Aging of polymers can potentially alter the performance of polymer-based therapeutics and therefore an understanding of the impact of aging on microsphere structure is important. Correlative focused ion beam […]Andrew G Clark
- Inhibition of bromodomain and extra-terminal (BET) proteins with JQ1 exacerbates acetaminophen-induced hepatotoxicity by altering detoxification pathways and oxidative stress responses March 29, 2025Acetaminophen (APAP) is a widely used over-the-counter drug for the treatment of fever and pain. At therapeutic doses, APAP has a relatively safe profile. However, at supratherapeutic doses it can produce liver injury and even fatal hepatotoxicity. Antioxidant genes regulated by the Kelch-like ECH-associated protein 1 (KEAP1)-nuclear factor, erythroid 2-like 2 (NRF2) pathway play a […]A P Laddha
- Conformational Analyses of the AHD1-UBAN Region of TNIP1 Highlight Key Amino Acids for Interaction with Ubiquitin March 28, 2025Tumor necrosis factor ɑ (TNFɑ)-induced protein 3 (TNFAIP3)-interacting protein 1 (TNIP1) is genetically and functionally linked to limiting auto-immune and inflammatory responses. We have shown that TNIP1 (alias A20-binding inhibitor of NF-κB 1, ABIN1), functioning as a hub location to coordinate other proteins in repressing inflammatory signaling, aligns with biophysical traits indicative of its being […]Michael L Samulevich
- Structural Insights into the Nonmutagenicity of 2-Haloacetophenone March 27, 2025A wide variety of endogenous and exogenous alkylating agents covalently modify DNA to produce N7-alkyl-2'-deoxyguanosine (N7-alkylG) adducts as major DNA lesions. The mutagenic potentials of many N7-alkylG adducts with an intercalatable moiety remain poorly understood. We have discovered that the antiriot agent 2-chloroacetophenone readily reacts with dG to produce N7-acetophenone-dG adducts, implicating the genotoxic properties […]Hunmin Jung
- Molecular Insight into Lipid Nanoparticle Assembly from NMR Spectroscopy and Molecular Dynamics Simulation March 26, 2025Lipid nanoparticles (LNPs) have emerged as the premier drug delivery system for oligonucleotide vaccines and therapeutics in recent years. Despite their prosperous advancement in research and clinical applications, there is a significant lack of mechanistic understanding of the assembly of lipid particles at the molecular level. In our study, we utilized a combination of solution […]Mingyue Li
- Impact on efficacy of target reduction of two FDA-approved ASO drugs by intracellular glucose levels in <em>in vitro</em> cell models March 19, 2025Antisense oligonucleotides (ASOs) have emerged as a new therapeutic modality for the treatment of both rare and common human diseases. A significant proportion of the patient population that may benefit from ASO therapy may also have common diseases, such as diabetes mellitus. The potential influence of prevalent diseases on the effectiveness of ASO drugs in […]Le Tra Giang Nguyen
- Protocol Development for CRISPR/Cas9 Knockout of the Anti-inflammatory Protein TNIP1 in HaCaT Keratinocytes March 19, 2025Gene editing in cultured cells, including the intent of sequence disruption to achieve a functional knockout of the targeted gene, has been greatly facilitated with the advent of Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) technology. Primary cell strains and immortalized cell lines from diverse tissue types have been successfully targeted both […]Liam E Carman
- Investigation of structural and dynamic properties of the Butyrophilin BTN3A1/BTN2A1 cytoplasmic complex by <sup>19</sup>F solution NMR March 13, 2025Butyrophilin 3A1 (BTN3A1) is an integral membrane protein capable of detecting phosphoantigens, like (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate (HMBPP), through its internal B30.2 domain. Detection of phosphoantigens leads to interactions with butyrophilin 2A1 and the subsequent activation of γδ-T cells. Though crystallography and functional assays have been crucial for determining vital residues of the BTN3A1/HMBPP/BTN2A1 complex, the mechanism […]Khiem Nguyen
- PVR exposure influences the activation, adhesion, and protein expression of human CD8+ T cells, including the CD96-mediated transfer of PVR March 12, 2025Poliovirus receptor (PVR) ligands have gained attention as immunotherapy targets, yet their regulation remains unclear. Here, we examine the impact of PVR exposure on primary human CD8+ T cells. We used flow cytometry and Western blot analysis to quantify expression of PVR and its ligands in naïve and effector T cells and used adhesion assays […]Xueting Huang
- Role of HNF4A-AS1/HNRNPC-mediated HNF4A ubiquitination protection against ritonavir-induced hepatotoxicity March 4, 2025Ritonavir (RTV) is an important drug for anti-human immunodeficiency virus treatment and is mainly metabolized by cytochrome P450 (CYP) 3A4. Clinically, the most common side effect of RTV treatment is hepatoxicity. We previously showed that the long noncoding RNA hepatocyte nuclear factor 4 alpha (HNF4A) antisense 1 (HNF4A-AS1) negatively regulated CYP3A4 expression and participated in […]Xiaofei Wang
- Impact of agglomeration on in vitro performance of long-acting injectable suspensions February 26, 2025Long-acting injectable (LAI) suspensions are controlled drug delivery systems that provide sustained drug release over durations ranging from one to six months. Although it is recognized that the particle agglomeration behavior could play a critical role on product clinical performance, factors that can affect this behavior have not yet been fully understood. This study explores […]Nilesh Malavia
- Advanced Manufacturing, Modeling And Analytical Tools In Injectable Products February 26, 2025Although great progress has been made in the effectiveness and availability of medicines, there is still more work to do assuring greater access and continuing to advance disease treatments. Recent advances in both the types of treatments that can be developed and the technology to produce, characterize, collect relevant data from, and deliver medicines to […]Howard Stamato
- Synthesis and evaluation of triazole-containing aryl/acyloxy prodrugs of a BTN3A1 ligand February 7, 2025The most effective natural ligand for the butyrophilins is (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate. However, due to its susceptibility to plasma hydrolysis and its high charge that limits passive diffusion across cell membranes, its potential as a drug is limited. Our efforts to identify compounds that stimulate γδ T cell proliferation have been focused on phosphonates to gain […]Umed Singh
- Optimization of Carrier-Based Dry Powder Inhaler Performance: A Review January 25, 2025Dry powder inhalers (DPI's) are becoming increasingly popular due to growing interest in pulmonary drug delivery and their performance is the net result of a series of processes carried out during the formulation development and manufacturing process such as excipient selection, blending, milling, filling, and spray drying. To reach the small airways of the deep […]Tanu Mehta